Lab report carbohydrate fermentation

Because the microorganism did not produce acidic end products that decrease the pH of the broth, thus the color of pH indicator bromcresol purple does not turn yellow. Durham tubes are inserted upside down in the test tubes to detect gas production.

Sugar fermentation test pdf

The test tubes were labeled 1, 2, 3, and 4. Carbohydrate fermentation patterns are useful in differentiating among bacterial groups or species. Tube 4 had more yeast than glucose while tube 3 had more glucose than yeast. The disaccharide was complex and we assumed there were no enzymes in the yeast to break down galactose. The raise in pH was being detected by an indicator known as bromothymol blue which change color from green to blue when the condition is alkaline. Sometimes, even though there is gas bubbles formed, the microorganism may produces non-acidic end products. Reference Hands-on-Labs. Nitrous oxide, nitrogen gas, hydrogen sulfide or methane might be produced and collected. This means that tube B shows a positive result. As for the V. Both experiments worked with yeast and a sugar. Developing our skill lab 2. The first experiment, experiment A, studied the fermentation exclusively between yeast and glucose. The product rely on the ability of the microorganism is skilled to carry out. Next the test tubes for the experiment were prepared.

This indicates that the bacterium grow and able to utilize the citrate as a carbon source. Normally, especially in aerobic respiration, the end products contain carbon dioxide.

Fermentation of carbohydrates reaction

Common end-products of bacterial fermentation include lactic acid, formic acid, acetic acid, butyric acid, butyl alcohol, acetone, ethyl alcohol, carbondioxide and hydrogen. A positive reaction is shown in the broth containing microorganism A while negative reaction is shown for the microorganism B. In citrate utilization test, our main purpose is to test the ability of a bacterium to utilize sodium citrate as a carbon source. Therefore, not only carbon dioxide may found in the air bubbles, other gases like nitrous oxide, nitrogen gas, hydrogen sulfide and methane gas could be found as well. Then, we recorded the results. A positive test was indicated by a blue color and negative test was indicated by a green color. This means that the condition of the agar is becoming alkaline. Fructose, a 5-Carbon sugar, was not taken into much consideration in the amount of carbon dioxide production; however we did believe that some CO2 would evolve from the combination.

Tube 4 had produced the most CO2, while tubes 1 and 2 showed almost no production. We felt that it may take more energy to break down the sugar. The test depends on the digestion of glucose to acetylmethylcarbinol. Table 1.

carbohydrate fermentation definition

As soon as the five minutes had passed, the respirometers were added to each test tube and made ready for obtaining data.

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Carbohydrate Fermentation Test: Uses, Principle, Procedure and Results